Abstract
The purpose of this study was to evaluate the applicability of online flow cytometry for assessing the adsorption process in surface water treatment. The research was conducted under real operating conditions at a plant treating up to 100,000 m³/day using coagulation, sedimentation, filtration, ozonation, adsorption (on WG-12 activated carbon), and disinfection. Daily water samples were collected from August to November 2024, including a period of raw water deterioration due to floodwater. Flow cytometric analysis was performed online every 30 minutes to determine microbial viability and activity. Findings revealed increased TOC removal efficiency with higher organic content, but also elevated microbial release from the adsorption bed during peak contamination. Flow cytometry effectively distinguished between microbial activity levels and responded faster than traditional turbidity monitoring. This method offers practical benefits for real-time water quality control and disinfection management. The study demonstrates the originality and value of integrating online cytometry into adsorption process monitoring in water treatment.
References
Alim, M. A., Rahman, A., Tao, Z., Samali, B., Khan, M. M., & Shirin, S. (2020). Suitability of roof harvested rainwater for potential potable water production: A scoping review. Journal of cleaner production, 248, 119226. https://doi.org/10.1016/j.jclepro.2019.119226
Anumol, T., Sgroi, M., Park, M., Roccaro, P., & Snyder, S. A. (2015). Predicting trace organic compound breakthrough in granular activated carbon using fluorescence and UV absorbance as surrogates. Water research, 76, 76-87. https://doi.org/10.1016/j.watres.2015.02.019
Besmer, M. D., Epting, J., Page, R. M., Sigrist, J. A., Huggenberger, P., & Hammes, F. (2016). Online flow cytometry reveals microbial dynamics influenced by concurrent natural and operational events in groundwater used for drinking water treatment. Scientific Reports, 6, 38462. https://doi.org/10.1038/srep38462
Claveau, L., Hudson, N., Jeffrey, P., & Hassard, F. (2024). Assessing microbial growth in drinking water using nucleic acid content and flow cytometry fingerprinting. iScience. 27(12), 111511. https://doi.org/10.1016/j.isci.2024.111511
Dao, M. U., Hoang, H. Y., Tran, A. K., & Cong, H. H. (2021). Assessment and treatment of floodwater in the Vietnamese Mekong Delta using a simple filter system based on silver nanoparticles coated onto activated carbon derived from rice husk. RSC advances, 11(63), 39838-39847. HTTPS://DOI.ORG/10.1039/D1RA06722B
Dos Santos, P. R., & Daniel, L. A. (2020). A review: organic matter and ammonia removal by biological activated carbon filtration for water and wastewater treatment. International journal of environmental science and technology, 17(1), 591-606. HTTPS://DOI.ORG/10.1007/s13762-019-02567-1
Dowdell, K. S., Olsen, K., Paz, E. F. M., Sun, A., Keown, J., Lahr, R., & Raskin, L. (2024). Investigating the suitability of online flow cytometry for monitoring full-scale drinking water ozone system disinfection effectiveness. Water Research, 257, 121702. https://doi.org/10.1016/j.watres.2024.121702
Goel, C., Mohan, S., & Dinesha, P. (2021). CO2 capture by adsorption on biomass-derived activated char: A review. Science of The Total Environment, 798, 149296. https://doi.org/10.1016/j.scitotenv.2021.149296
Harja, M., Buema, G., & Bucur, D. (2022). Recent advances in removal of Congo Red dye by adsorption using an industrial waste. Scientific Reports, 12(1), 6087. https://doi.org/10.1038/s41598-022-10093-3
Huang, Z., Yuan, X., & Liu, X. (2021). The key drivers for the changes in global water scarcity: Water withdrawal versus water availability. Journal of Hydrology, 601, 126658. https://doi.org/10.1016/j.jhydrol.2021.126658
Li, C. Y., Huang, T. L., Wen, C. C., Liang, W. G., Lin, Z. S., Yang, S. Y., ... & Cai, X. C. (2021). Influence of storm runoff on the spectral characteristics of dissolved organic matter (DOM) in a drinking water reservoir during the flood season, 42(3), 1391-1402. https://doi.org/10.13227/j.hjkx.202007276
Lim, K. Y., & Foo, K. Y. (2021). Hazard identification and risk assessment of the organic, inorganic and microbial contaminants in the surface water after the high magnitude of flood event. Environment international, 157, 106851. https://doi.org/10.1016/j.envint.2021.106851
Lu, Z., Jing, Z., Huang, J., Ke, Y., Li, C., Zhao, Z., & Sun, W. (2022). Can we shape microbial communities to enhance biological activated carbon filter performance? Water Research, 212, 118104. https://doi.org/10.1016/j.watres.2022.118104
Mahmud, F. (2024). Removal of Phosphorous, Copper and Zinc from Stormwater Using Powdered Activated Carbon (PAC) Based Water Treatment Residuals. South Dakota State University. https://www.proquest.com/docview/3052288183?fromopenview=true&pq-origsite=gscholar&sourcetype=Dissertations%20&%20Theses
Mishra, A., Alnahit, A., & Campbell, B. (2021). Impact of land uses, drought, flood, wildfire, and cascading events on water quality and microbial communities: A review and analysis. Journal of Hydrology, 596, 125707. https://doi.org/10.1016/j.jhydrol.2020.125707
Piai, L., Mei, S., van Gijn, K., & Langenhoff, A. (2024). Effects of organic matter in drinking water and wastewater on micropollutant adsorption to activated carbon. International Journal of Environmental Science and Technology, 21(3), 2547-2558. https://doi.org/10.1007/s13762-023-05132-z
Ponting, J., Kelly, T. J., Verhoef, A., Watts, M. J., & Sizmur, T. (2021). The impact of increased flooding occurrence on the mobility of potentially toxic elements in floodplain soil–A review. Science of the Total Environment, 754, 142040. https://doi.org/10.1016/j.scitotenv.2020.142040
Rosińska, A., & Dąbrowska, L. (2021). Influence of type and dose of coagulants on effectiveness of PAH removal in coagulation water treatment. Water Science and Engineering, 14(3), 193-200. https://doi.org/10.1016/j.wse.2021.08.004
Rowles III, L. S., Hossain, A. I., Ramirez, I., Durst, N. J., Ward, P. M., Kirisits, M. J., Ariza, I., Lawer, D. F., & Saleh, N. B. (2020). Seasonal contamination of well-water in flood-prone colonias and other unincorporated US communities. Science of the Total Environment, 740, 140111. https://doi.org/10.1016/j.scitotenv.2020.140111
Si, F., Huang, T., Li, N., Li, K., Wen, G., Li, Y., & Zhang, H. (2022). Effects of flood discharge on the water quality of a drinking water reservoir in China–Characteristics and management strategies. Journal of Environmental Management, 314, 115072. https://doi.org/10.1016/j.jenvman.2022.115072
Steadmon, M., Ngiraklang, K., Nagata, M., Masga, K., & Frank, K. L. (2023). Effects of water turbidity on the survival of Staphylococcus aureus in environmental fresh and brackish waters. Water Environment Research, 95(9), e10923. https://doi.org/10.1002/wer.10923
Su, Y., Gao, R., Huang F., Liang, B., Guo. J., Fan, L., & Wang, A. (2024). Occurrence, transmission and risks assessment of pathogens in aquatic environments accessible to humans. Journal of Environmental Management, 354, 120331. https://doi.org/10.1016/j.jenvman.2024.120331
Teplykh, S. Y., Kotovskaya, E. E., & Gorshkalev, P. A. (2024). Improving the technology of drinking water purification at the water treatment facilities of the Chernorechensk reservoir during the flood period. Urban construction and architecture, 14(3), 49-59. https://doi.org/10.17673/Vestnik.2024.03.06
Radovanović, S. A., Mihailović, N. R., Radovanović Ž. M., & Veljković, L. M. (2023). Flood frequency analysis in the world. Tokovi osiguranja, 39(3), 367-388. https://doi.org/10.5937/TokOsig2303367R
Wang, Z., Li, Y., Hu, M., Lei, T., Tian, Z., Yang, W., ... & Graham, N. J. (2022). Influence of DOM characteristics on the flocculation removal of trace pharmaceuticals in surface water by the successive dosing of alum and moderately hydrophobic chitosan. Water Research, 213, 118163. https://doi.org/10.1016/j.watres.2022.118163
Wilkinson, M. G. (2018). Flow cytometry as a potential method of measuring bacterial viability in probiotic products: A review. Trends in food science & technology, 78, 1-10. https://doi.org/10.1016/j.tifs.2018.05.006
Yang, J., Han, Z., Yan, Y., Guo, G., Wang, L., Shi, H., & Liao, X. (2024). Neglected pathways of heavy metal input into agricultural soil: Water–land migration of heavy metals due to flooding events. Water Research, 267, 122469. https://doi.org/10.1016/j.watres.2024.122469

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Copyright (c) 2026 Economics and Environment
